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Cell lysis method to get "intact" nuclear protein complex for CO-IP?

cell lysis Co-IP protein complex

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#1 asaren

asaren

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Posted 13 May 2015 - 05:34 PM

I would like to find cell lysis method to solubilize/break-up nuclear membrane without breaking/denaturing protein-protein interaction of protein complex in nucleus before doing CO-IP. I need to find method to breakup nuclear membrane, however It seems that RIPA buffer and sonication method are not recommend which can denature protein-protein interaction. Could you recommend me a method to break up nuclear membrane to get "intact" nuclear protein complex for CO-IP? especially chemical method.

 

Thank you so much



#2 bob1

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Posted 14 May 2015 - 12:02 AM

I use a buffer called IPPT (based on this paper) for RB.







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