Dear all,
I wonder if anybody ever tried to do bisulfite conversion with Ampure XP beads (SPRI) in the solution?
I know zymo research has a magbeads protocol. But according to zymo their beads have no size selection properties.
What I would like to do is a protocol similar that of Fisher et al. http://genomebiology...content/12/1/R1
keeping all steps on Ampure XP beads... (have very very little DNA...)
So fragmentation or restriction of gDNA, end-repair, adapter ligation, size selection of >150bp, bisulfite conversion, cleanup, desulfanation,cleanup
best