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#1 Chomolungma

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Posted 12 May 2015 - 02:44 AM

Hi all, I got well amplified non specifically annealed bands in my GM testing PCR at 58.4 annealing temperature, even at 0.2 microliter volume of primers in a 15 microliter reaction volume. I also got  the gm positive band along with that. what can I do to prevent it?

Thanks 



#2 bob1

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Posted 14 May 2015 - 12:04 AM

Contamination? Try different temperatures/conditions?






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