Does ultrasonic probe sonication break up mitochondria or nuclear membrane of cultured cell?
I am confused about this for long time. The ultrasonic probe sonication is used in some paper to homonoginize cell lysate then centrifuge to isolate intact mitochondria (no breaking mitochondrial membrane). However many guides in google search also mention that the sonication can break every organelle membrane. Why is so different? Is it about the power setting or time period to sonicate cell on ice?
In addition, Does the sonication disassociate protein-protein interaction in CO-IP?
Thank you very much