Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
- - - - -

Buffer and method for cell fractionation (mitochondrial, nuclear, cytosol protei

cell fractanation mitochondria isolation nuclues isolation cell lysis

  • Please log in to reply
No replies to this topic

#1 asaren



  • Active Members
  • Pip
  • 22 posts

Posted 09 May 2015 - 05:54 PM

Hello Guys,


I have some questions regarding to buffer and method for cultured cell fractionation to get mitochondrial, nuclear, cytosol proteins as following:

  1. Can I just use PBS with some inhibitors for buffer? because what matter is centrifuge speed to dissociate cell organelles.
  2. Can I use just mild lysis buffer (NP40 or tritonx100) to homoginize cell lysate instead of douce homoginizer before centrifuge each pellet?
  3. There are so many recipe for the buffer and method for cell fractanation, which is the best or most standard protocol I should follow? Any paper suggestion?

Thank you so much

Edited by asaren, 09 May 2015 - 08:11 PM.

Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.