Does ultrasonic probe sonication break up mitochondria or nuclear membrane?
I read from some forum post that it can break up mitochondria or nuclear membrane with high frquency. What is the frquency? On contary, many papers use the sonication method to isolate intact mitochondria (no breaking mitochondrial membrane).
In addition, If I want to collect protein for CO-IP, Can I do the sonication with cell lysate?
Thank you very much
Edited by asaren, 09 May 2015 - 09:39 AM.