
Effect of reaction volume on enzyme activity?
#1
Posted 06 May 2015 - 12:41 AM
- Sara Abdou likes this
#2
Posted 06 May 2015 - 03:24 AM
is it concentration of enzyme and substrate or amount added that are kept constant?
if amount then you have a higher concentration, resulting in greater activity.
if concentration then the greater activity could be due to greater uniformity and more rapid equilibration of assay conditions with lower volume.
- squallweathered and Sara Abdou like this
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#3
Posted 06 May 2015 - 10:46 AM
Small volumes can sequester DNA or protein or both on the side walls of tubes. Perhaps this is the issue here.
- squallweathered and Sara Abdou like this
#4
Posted 11 May 2015 - 06:02 AM
Many thanks mdfenko and phage434.
is it concentration of enzyme and substrate or amount added that are kept constant?
We scale down the added volumes by 20x of all reagents, so the amount added is reduced, but the concentration of each relative to each other is maintained.
Thanks for all advise.
Also tagged with one or more of these keywords: enzyme activity, restriction enzyme
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