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Agarose Gel Electrophoresis


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#1 Sakumi

Sakumi

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Posted 05 May 2015 - 04:46 PM

Hi All,

 

I am still doing the old fashioned PCR where I run my products on gel and then image them under UV to analyze expression levels.

 

I am taught to run my gel for 60min at 60V. But I'm wondering, if I know which gene is loaded into which well, do I need to run the full 60min to just get the bands so I can analyze them?

 

In other words, if I just want to analyze the expression levels, can I just run for 20min (for example) so the bands form?

 

Thank you!

 

Sakumi



#2 phage434

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Posted 05 May 2015 - 06:09 PM

Of course.






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