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Measuring IPSPs in a pacemaker neuron

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#1 Sakumi



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Posted 05 May 2015 - 12:33 PM

Hi guys,


I am trying to measure IPSPs in a cell using current clamp. The cell does not have a stable membrane potential, but rather an oscillating membrane potential, because it is a pacemaker neuron. But the oscillation is not big, a few millivolts at most.


I am getting some results, but I just feel so uncertain because I can't use voltage clamp and control the membrane potential.


Has anyone done anything similar? Like measuring PSPs using current clamp?


Thank you!



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