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Measuring IPSPs in a pacemaker neuron


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#1 Sakumi

Sakumi

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Posted 05 May 2015 - 12:33 PM

Hi guys,

 

I am trying to measure IPSPs in a cell using current clamp. The cell does not have a stable membrane potential, but rather an oscillating membrane potential, because it is a pacemaker neuron. But the oscillation is not big, a few millivolts at most.

 

I am getting some results, but I just feel so uncertain because I can't use voltage clamp and control the membrane potential.

 

Has anyone done anything similar? Like measuring PSPs using current clamp?

 

Thank you!

 

Sakumi






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