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cDNA library construction

cDNA library Nicotiana benthamiana

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#1 Leonor Bedoya

Leonor Bedoya

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Posted 05 May 2015 - 02:29 AM

I need to construct a cDNA library from Nicotiana benthamiana plants and use it in a VIGS screening to find plant factors thar interact with the PPV coat protein.

 

I have never done a library so I need help to know which is the best way, that is less expensive and faster way, to construct the cDNA library. Which kit do I use? In wich vector for E. coli? How to normalize?

 

Thanks!

 

 







Also tagged with one or more of these keywords: cDNA library, Nicotiana benthamiana

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