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Nalidixic acid adapted strains

Nalidixic acid

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#1 Dima

Dima

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Posted 01 May 2015 - 11:28 AM

Dear members,

 

could anyone help sharing a protocol on strains (E.coli or Salmonella) adaptation to Nalidixic acid? the idea is to separate the E.coli strains I am adding (inoculating in food) from the indigenous ones that may be present.

 

I started gradually until cells were grown in broth supplemented with Na (50microl/ml).It didnt seem to work as other background flora grew as well with my reference strains.

 

I would like to double check with others who might have been working on this before i keep increasing the Na concentration.

 

Hope to hear some comments.

 






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