Is there a cheap and safe replacement for EtBr in agarose gels for visualizing nucleic acid - DNA and RNA? Specifically a dye that you can add to both running buffer and the gel which is how I use EtBr. I do not want to do a post stain because I am too impatient to wait and I occasionally like to view my gel before it has fully resolved. I have heard that these dyes are large and can alter the migration of the nucleic if they are added to the gel so I am not sure if they will all require a post stain. However, I am not aware of all the dyes available or what other people are doing in their labs. I want to get rid of EtBr because of the special requirements for disposal. Plus I would like a dye that I can use for both UV and blue light in case I do not want to expose my DNA to UV. So I was hoping someone had some success with the switch form EtBr to a safe dye.