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Lentivirus


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#1 Lee6948

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Posted 29 April 2015 - 03:35 AM

Hi everyone:

 

Recently I've been using puromycin to selected cells which were transduced with lentiviral vectors and I had added puromycin 3 times already.

Should I still use this drug to screen for successfully transduced cells or move on to my experiments with such cells?

 

                                                                                          Thanks a bunch

 

                                                                                                                                                          Dean



#2 bob1

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Posted 29 April 2015 - 03:38 PM

You first need to prove that the transduction has worked. Perhaps flow cytometry or immunofluorescence with staining for your target protein to check that the cells are all transduced.






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