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Tryple express vs scraping

protein expresssion

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#1 Peniel

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Posted 27 April 2015 - 04:55 AM

Hi all,

 

Please when performing a cell signalling experiment, which is the best method in harvesting the cells after treatment to have minimal damage to cell surface receptors.

 

Thanks for your help!



#2 bob1

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Posted 27 April 2015 - 01:50 PM

Harvest for what purpose? If you are just harvesting for western blotting, then lysis directly in the dish is probably the best.



#3 Peniel

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Posted 28 April 2015 - 12:40 AM

Sorry I left out the purpose. I am harvesting for Western blot and Flow cytometry. 

 

Many thanks. 







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