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Preferred assay for measuring cytotoxicity or cell proliferation

Cytotoxicity

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2 replies to this topic

#1 Peniel

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Posted 27 April 2015 - 02:18 AM

Hi all,

 

Please I would like to know the views of the experts here on the best method to measure cytotoxicity. I generally use MTS assay but I have come across a few papers that indicate it might not be sensitive enough and I don't want to be faulted based on that. So please experts share your views....

 

 

Cheers!



#2 Curtis

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Posted 27 April 2015 - 11:14 PM

I believe MTT is still the most popular method out there.



#3 DRT

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Posted 28 April 2015 - 12:06 PM

This webinar might be useful for you. They are pushing their own products but it gives a useful overview of how to link different types of assays to get a complete picture of cytotoxicity.

[ https://promega.adob...e&pbMode=normal ]

 

btw. I use PerkinElmer's ATPLite for general cytotox screening, despite it's high price, because I find it faster and gives tighter error bars.







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