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OD measurement

optical density growth culture

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#1 CristianaCris

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Posted 24 April 2015 - 04:07 AM

Hello everyone!

I want to measure the OD of 5 anoxic bacteria culture which have different substrate. I do this for obtaining the growth curve. I mesured the OD but I had some wrong results so I freeze the first 3 samples and I measured by spectophotomer again and the values were what I was expecting. I did the same for another 2 days.

Now I thaw out the samples and when I measure the OD of all and I have absurde results.

What I did wrong or what I could do from now on?



#2 bob1

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Posted 27 April 2015 - 12:32 AM

Freeze thaw cycles will damage some of your cells leading to release of cellular components, which means that frozen samples will have at best inaccurate readings, at worst readings that don't mean anything at all as the cell components absorb in the same range as bacteria.



#3 CristianaCris

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Posted 30 April 2015 - 04:13 AM

Thank you!






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