Please help me.
I performed E. coli transformation by heat shock.
But I have no colony.
My condition is as follows.
- E. coli cp cell, 200 ul
- 18E7 plasmid(1.4 ug/ml), 10 ul
- Amp+(50 ug/ml) LB plate
The portions of plasmid is too small?
Or I have another problems?