I've received a small amount of plasmid DNA that is a mammalian expression vector. There isn't any bacterial antibiotic selection, only eukaryotic. Before I conduct the transfection, I want to generate sufficient DNA for repeats. If I were to transform the plasmid into bacteria, would the cells be able to replicate the plasmid DNA for mini/maxiprep?
There is an SV40 promotoer, but my understanding is that only eukaryotes can read this.