I have a question. I want to measure the expression levels of some proteins. Here in my lab, people usually do western, but it is not good as a quantitative method and is also a little expensive (if you buy the reagents).
I never worked with ELISA, but this method may be a good choice to evaluate the expression levels of some proteins in my project, it can evaluate a lot of samples at the same time, is cheaper and seems to be quantitative. Is it true? Does anyone with experience with elisa and western can help me to clarify these questions?
Thank you very much guys!