New user here, and I am hoping to get some insight on a research problem we have been having in my lab. I have been working on determining the sequences of antibodies expressed in mouse hybridoma cell lines using a variety of primer sets targeting the heavy chain variable and light chain variable (Vk) regions. When sequencing the Vk region of one particular hybridoma, I consistently get 2 distinct sequences with my primer panel, despite doing limited dilutions and attempting to get a monoclonal population. This makes me think that this strain is polyclonal despite our efforts at doing limited dilutions, unless the sequences belong to the mouse germline. I've tried checking these sequences against known mouse germline and mouse pseudogene sequences but am not getting any matches. Has anyone here seen these sequences come up in their work as known mouse germline genes? If not, do you know of any good databases where I could try to match these sequences to the mouse germline?
Thanks in advance for your help! The amino acid sequences are below