I hope that someone could help me with the following issue:
I want to differentiate HL60 cells into granulocytes with DMSO and into monocytes/macrophages with TPA respectively.
which surface markers could I use to distinguish between the two lineages (and to assess successful differentiation)
So far I have:
granulocytes: CD24, CD66b
monocytes/macrophages: CD14, CD68, CD33
Does anyone have experience on this?
Thanks a lot!