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Which markers to distinguish granulocytes/monocytes (HL60)

HL60granulocytes monocytes macrophages FACS

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#1 EvelynMDC

EvelynMDC

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Posted 14 April 2015 - 11:14 PM

Hello,

 

I hope that someone could help me with the following issue:

 

I want to differentiate HL60 cells into granulocytes with DMSO and into monocytes/macrophages with TPA respectively.

 

which surface markers could I use to distinguish between the two lineages (and to assess successful differentiation)

 

So far I have:

 

granulocytes: CD24, CD66b

 

monocytes/macrophages: CD14, CD68, CD33 

 

both: CD11c

 

Does anyone have experience on this?

 

Thanks a lot!

 







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