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Which markers to distinguish granulocytes/monocytes (HL60)

HL60granulocytes monocytes macrophages FACS

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#1 EvelynMDC



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Posted 14 April 2015 - 11:14 PM



I hope that someone could help me with the following issue:


I want to differentiate HL60 cells into granulocytes with DMSO and into monocytes/macrophages with TPA respectively.


which surface markers could I use to distinguish between the two lineages (and to assess successful differentiation)


So far I have:


granulocytes: CD24, CD66b


monocytes/macrophages: CD14, CD68, CD33 


both: CD11c


Does anyone have experience on this?


Thanks a lot!


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