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Any other reagents could substitute DTT


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3 replies to this topic

#1 Tangel Lee

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Posted 02 August 2004 - 01:20 AM

Is there any reagents have similiar effect as DTT to break the covalent bonds in protein and need not special storage condition?

#2 Oleksii

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Posted 02 August 2004 - 07:01 AM

Hi,

You can use b-mercaptoethanol in concentration about 20 mM. And check "Pirce" catalog to find more substitution.

Oleksii

#3 tfitzwater

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Posted 03 August 2004 - 03:24 PM

Other replacements are TCEP (Tri(2-carboxyethyl)phosphine, hydrochloride) which is more stable and odorless, Meso-DTA (Meso-2,5-dimercapto-N, N, Ní, Ní-tetramethyladipamide), MESNA (Mercaptoethane sulphonic acid), and possibly NALC (N-acetyl-L-cysteine).

Edited by tfitzwater, 03 August 2004 - 03:24 PM.


#4 mpanchal

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Posted 06 August 2004 - 07:13 AM

you can use glutathion or cystein in place of DTT

Edited by mpanchal, 06 August 2004 - 07:14 AM.





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