Is there any reagents have similiar effect as DTT to break the covalent bonds in protein and need not special storage condition?
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Any other reagents could substitute DTT
Started by Tangel Lee, Aug 02 2004 01:20 AM
3 replies to this topic
#1
Posted 02 August 2004 - 01:20 AM
#2
Posted 02 August 2004 - 07:01 AM
Hi,
You can use b-mercaptoethanol in concentration about 20 mM. And check "Pirce" catalog to find more substitution.
Oleksii
You can use b-mercaptoethanol in concentration about 20 mM. And check "Pirce" catalog to find more substitution.
Oleksii
#3
Posted 03 August 2004 - 03:24 PM
Other replacements are TCEP (Tri(2-carboxyethyl)phosphine, hydrochloride) which is more stable and odorless, Meso-DTA (Meso-2,5-dimercapto-N, N, N’, N’-tetramethyladipamide), MESNA (Mercaptoethane sulphonic acid), and possibly NALC (N-acetyl-L-cysteine).
Edited by tfitzwater, 03 August 2004 - 03:24 PM.
#4
Posted 06 August 2004 - 07:13 AM
you can use glutathion or cystein in place of DTT
Edited by mpanchal, 06 August 2004 - 07:14 AM.