3 replies to this topic

[Tangel Lee]

Is there any reagents have similiar effect as DTT to break the covalent bonds in protein and need not special storage condition?

[Oleksii]

Hi,

You can use b-mercaptoethanol in concentration about 20 mM. And check "Pirce" catalog to find more substitution.

Oleksii

[tfitzwater]

Other replacements are TCEP (Tri(2-carboxyethyl)phosphine, hydrochloride) which is more stable and odorless, Meso-DTA (Meso-2,5-dimercapto-N, N, N’, N’-tetramethyladipamide), MESNA (Mercaptoethane sulphonic acid), and possibly NALC (N-acetyl-L-cysteine).

Edited by tfitzwater, 03 August 2004 - 03:24 PM.

[mpanchal]

you can use glutathion or cystein in place of DTT

Edited by mpanchal, 06 August 2004 - 07:14 AM.