Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Why does DMEM always contain 3.7g/L of sodium bicarbonate?

Culture Media Sodium Bicarbonate DMEM 3.7 g/L 5% - 10% CO2

  • Please log in to reply
1 reply to this topic

#1 mevcit

mevcit

    member

  • Members
  • Pip
  • 3 posts
0
Neutral

Posted 14 April 2015 - 04:30 AM

Hi everyone,

 

Companies formulate DMEM always with 3.7g/L of sodium bicarbonate. But majority of researchers use only 5% CO2 incubators, which is ideal for media with 1.5-2.2g/L of sodium bicarbonate. 3.7g/L of NaHCO3 requires 10% CO2. So, while most of us use only 5% CO2 incubators and everyone knows this, companies must have a good reason for insisting on 3.7g/L. Do you have an idea on this? Does DMEM have to contain 3.7g/L NaHCO3 for some reason?

 

People in my lab also use DMEM but they didn't know DMEM requires 10% CO2. I let them know this but they are not willing to change their culture conditions as all of their experiments have been done in 5% CO2 all the time. So I decided to buy DMEM with lower NaHCO3 concentration. I found one from ATCC (1.5g/L), but they have only high glucose DMEM (I use low glucose DMEM). I found powder forms where I can add the desired amount of NaHCO3 but I don't want to spend time on preparation, pH adjustments and sterility issues. Do you have a suggestion about what to do? Should I just ignore these questions in my mind and keep doing what many researchers have been doing wrongly (i.e. using DMEM with 5% CO2)?

 

Thanks in advance for your replies.


Edited by mevcit, 14 April 2015 - 04:32 AM.


#2 SusieQ

SusieQ

    Enthusiast

  • Active Members
  • PipPipPipPipPip
  • 40 posts
2
Neutral

Posted 23 April 2015 - 06:02 AM

I'm not sure if this is allowed, but I think you can find your answer here:

 

http://www.researchgate.net/post/Why_did_the_pH_level_of_DMEM_increase_when_placed_in_an_incubator_with_no_cells

 

From this I filter that you're right regarding the bicarbonate concentration and CO2 percentage. Perhaps it's just that this is the standard WoW, and nobody wants to deviate from this as it's 'how it's done'.


Edited by SusieQ, 23 April 2015 - 06:03 AM.






Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.