Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

How to achieve a uniform monolayer with HepG2 cells, seeded in 24 welll plate fo


  • Please log in to reply
1 reply to this topic

#1 attached to cells!

attached to cells!

    member

  • Members
  • Pip
  • 2 posts
0
Neutral

Posted 10 April 2015 - 06:17 PM

I tried plating HepG2 (passage 9) in 24 well plate format with cell seeding density of 4 X 10^5 cells /mL and added 500 uL in each well. But I always notice cells pool in the center. 



#2 Curtis

Curtis

    Metaller Scientist

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 1,163 posts
62
Excellent

Posted 10 April 2015 - 07:32 PM

This is a typical problem actually. To improve, don't swirl the plate when you seed. Instead you rotate the plate north to south, east to west , like a cross few times, and gently put in incubator. We find cells accumulate less in the center.






Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.