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method to detect 5ŽOH end of RNA


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#1 littleRNA90

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Posted 10 April 2015 - 02:43 AM

Hi everybody!

 

I want to detect the 5´ OH end of a specific mitochondrial RNA. Can someone help me out?

 

Thanks in advancesmile.png



#2 phage434

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Posted 10 April 2015 - 12:28 PM

Typical mitochondrial RNA will have a 5' triphosphate. What makes you think it will have a 5' OH?



#3 littleRNA90

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Posted 12 April 2015 - 11:37 PM

you are right but there is one processing site that is known to have a 5 OH






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