For my class I have to summarize few papers (like journal club) and I have one question about the method.
From the paper, "A Synthetic Genetic Edge Detection Program" (http://www.sciencedi...2867409005091), there is a detailed protocol "Quick Edge Detection Protocol" in supplemental data. In step 11, is it saying pour inoculated agarose solution with cells into EMPTY Petri Dish? Or is it assuming you have already prepared solid layer of inoculated agarose solution without cells in Petri Dish that you pour solution with cells on top of the solid layer?
I am new to microbiology so I don't know if preparing agar plate is so basic that they omit such step or not.
Tabor, Jeffrey J., et al. "A synthetic genetic edge detection program." Cell 137.7 (2009): 1272-1281.
Edited by anonyvous, 09 April 2015 - 03:34 PM.