Could you please help me if you have any idea about my results :
I isolated lactic acid bacteria from cattles sample. Then i grew them with MRS agar in anaerob environment. I was very careful about contamination.
I choosed two potential isolates. After that I did biochemical identification using API 50 CHL kit and molecular identification using 16S rRNA.
But then i found my two isolates gave different results.
1st Isolat : API - Lactococcus lactis 78%; 16S - Enterococcus sp. 99%
2nd isolat : API - Lactobacillus fermentum 96% ; 16S - Streptococcus sp. 99%
Just, how come??? I don't understand.
Why it gave different results even though it's from same culture?
The 16S rRNA identification didn't give the information about species but only as sp. The 2nd isolats even more confusing because one is bacils and one is coccus. And i'm not sure either if it's coccus or bacil..i'm only sure if it chains in microscope.
I'm so curious and no one near me could help me about what's actually going on.
Thank you very much.
Looking forward to hear your suggestion.