Sorry to hijack this thread but I rather not make another thread about Infusion.
I am trying to use Infusion for the first time to do some cloning. I made primers for my insert with the Infusion online tool, did the PCR and verified it by running it on a gel. Is it possible to do the infusion step with the PCR product as is? I gel purified my insert but I ended up recovering <300ng total and there's a lot of salt in it (low 260/230 but good 260/280).
I linearized my vector tonight and deactivate the RE. Can I skip the gel purification step and use whatever amount of vector I need after I nanodrop it again? I am sure Ill lose a lot of the vector and will and up with a shitty 260/230 ratio.
Btw, anyone knows approximately how much DNA you get from a 30-34 cycles reaction?It should be a few micrograms right?
Edited by Wek, 30 April 2015 - 09:38 PM.