I've been trying to detect the chicken IP3R in a Western Blot, but since it's a kind of heavy protein, ca. 320 kDa, I've had no success. I am reviewing all the conditions and wanted to know what would be the adequate %gel, voltage and running time to discern clearly the protein in the gel. The transference onto the membrane is another step, about which I'll ask later. Please help me out! Thanks in advance.