I am writing my bachelors thesis and I am a bit stuck and feel unsure.. . I am looking at activity of few metabolic enzymes in fish larvae (Citrate Synthase and Pyruvate Kinase). Since my extracts are not pure I have to calculate specific enzyme activity. The enzyme activities are in U/g wet mass (U = µmol min-) and protein content is in µg/mg. So I have to divide enzyme activity by protein content..right? but I am not sure of the final units.
Here is an example for one sample for the enzyme Citrate synthase:
Enzyme activity = 6.728 U/g
Protein content = 24.222 µg/mg
Do I have to do a conversion of one of the factors to have uniform units?..how?
Is someone kind enough to sort out the units for the above as an example?...any useful sources where I can read on such a topic?
I will really appreciate your contributions
Edited by Mwanafunzi, 04 April 2015 - 06:58 AM.