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GFP interference in dual luciferase assay?

dual-luciferase gfp lipofectamine 2000

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#1 adsc

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Posted 02 April 2015 - 01:56 AM

Dear colleagues,

 

I performed a GFP transfection test on a difficult-to-transfect cell line with Lipofectamine 2000, can I use these cells (still expressing GFP) to perform my dual-luciferase experiments? Should I wait until they stop expressing GFP in case it interferes in the luminometer? Or would thawing out new ones be the safest?

 

Thank you in advance - A

 



#2 pavoni.ernesto

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Posted 02 April 2015 - 04:37 AM

Hi,

find the post in the Archive Cell Biology:  "GFP constructs in Luciferase assay" posted Mar/16/2010



#3 adsc

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Posted 02 April 2015 - 05:04 AM

cheers!







Also tagged with one or more of these keywords: dual-luciferase, gfp, lipofectamine 2000

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