Posted 02 April 2015 - 02:43 PM
Personal advice is, don't get the older model akta fplc now If you are going to use it for long term. GE had phased it out and the service parts is heard will be fully discontinued on 2017, this is what had been communicated in my region.
They had replaced their product line with akta start (very beginner entry level, cost around 10k USD end user selling price, maximum flow rate 5ml/min peristaltic pump) , akta pure, flow rate maximum 25 or 150ml/min (lab scale, replacing akta purifier and akta fplc) or akta avant (Pre-pilot scale, replacing akta explorer)series, with same flow rate with akta pure.
Depending on the expected usage(preparative or analytical scale), different configuration will be suitable for different users. Personally I would get configuration to install column selection valve, where I can install up to 5 or 6 columns with a column bypass line, which will be easier when trying to optimize column selection using the method queue (queue up different method in one shot, just press run and will run the column screening for you). On top, I might install additional valve so that I can hook up with more inlet buffers, but yet that depends on the nature of your work and what exactly you are purifying. And if you are expecting your purification step to be fully automated into two steps purification, you will need extra valve to perform the flow back. Finally, you would look into the flow rate. Higher flow rate (150ml/min) enables to use the axichrome automated column packing(with column valve installed to enable such function), while lower flow rate systems (25ml/min, except akta start) gives you high screening and optimization precision. I would for sure get a superloop for variable sample loading sizes.
Just my two cents for now.
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