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Differences between DH5a strain of E. Coli and BL21 strain of E.Coli

Started by Burak Kaan Yasdı, Apr 01 2015 06:37 AM

2 replies to this topic

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Posted 01 April 2015 - 06:37 AM

Can anybody inform me about differences between DH5a strain of E. Coli and BL21 strain of E.Coli

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Posted 01 April 2015 - 09:21 AM

Can anybody inform me about differences between DH5a strain of E. Coli and BL21 strain of E.Coli

Both links can be usefull..

Its hard to tell you what the differences are that matter for you...

In short: DH5a is a general strain for cloning and blue white screening
BL21 is more for protein expression, it lacks certain proteases.

If you don't know it, then ask it! Better to ask and look foolish to some than not ask and stay stupid.

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Posted 01 April 2015 - 02:45 PM

Thanksss. This is a homework from my molecular biology laboratory class.