Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Differences between DH5a strain of E. Coli and BL21 strain of E.Coli


  • Please log in to reply
2 replies to this topic

#1 Burak Kaan Yasdı

Burak Kaan Yasdı

    member

  • Active Members
  • Pip
  • 15 posts
0
Neutral

Posted 01 April 2015 - 06:37 AM

Can anybody inform me about differences between DH5a strain of E. Coli and BL21 strain of E.Coli



#2 pito

pito

    Veteran

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 1,479 posts
101
Excellent

Posted 01 April 2015 - 09:21 AM

Can anybody inform me about differences between DH5a strain of E. Coli and BL21 strain of E.Coli

 

 

https://bio.as.uky.e...s_genotypes.pdf

http://openwetware.o..._coli_genotypes

 

Both links can be usefull..

 

Its hard to tell you what the differences are that matter for you...

In short: DH5a is a general strain for cloning and blue white screening
BL21 is more for protein expression, it lacks certain proteases.


If you don't know it, then ask it! Better to ask and look foolish to some than not ask and stay stupid.


#3 Burak Kaan Yasdı

Burak Kaan Yasdı

    member

  • Active Members
  • Pip
  • 15 posts
0
Neutral

Posted 01 April 2015 - 02:45 PM

Thanksss. This is a homework from my molecular biology laboratory class. 






Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.