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How to obtain a control positive for my WB

Control +ve for WB

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#1 Mohamed 1984

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Posted 01 April 2015 - 03:46 AM

I am detecting Influenza protein (NP) in a set of samples. I would like to have a control +ve for my WB. Now I thought to clone the NP gene in a plasmid that express it. But how to show the protein of the NP in WB ? 

 

Could you list some methods as to how to get a Ctr +ve for my WB ? 



#2 bob1

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Posted 01 April 2015 - 12:12 PM

If you can express if off a plasmid and can get (or make) an antibody against the protein, that you can then show detects a band of the correct size and can be competed by peptide, then that is usually considered sufficient.



#3 BMF

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Posted 01 April 2015 - 12:20 PM

this is a little off topic:

 

http://www.ncbi.nlm....les/PMC3840294/



#4 Mohamed 1984

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Posted 02 April 2015 - 01:53 AM

Thanks






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