Javascript Disabled Detected

You currently have javascript disabled. Several functions may not work. Please re-enable javascript to access full functionality.

Submit your paper to J Biol Methods today!

Co-IP Pierce Kit - protocol questions

Started by VBlack , Mar 31 2015 03:22 AM

Co-IP

No replies to this topic

member

0
Neutral

Posted 31 March 2015 - 03:22 AM

Hello,

I have been using the Pierce Co-IP Kit (number 26149) and I am bit stuck with the protocol and specifically with step D.

I have done only the step A so far-immobilised my antibody.

I was wondering is there anybody here that has some experience with this protocol so maybe I can ask some (probably stupid) questions?

Thanks

Protocols and Techniques Forums → SDS-PAGE and Western Blotting → Does the diameter of magnetic protein A or G beads matter for immunoprecipitatio Started by 5280 , 18 Mar 2016 Magnetic protein G beads and 4 more...	0 replies 1,555 views	5280 18 Mar 2016
Protocols and Techniques Forums → Protein and Proteomics → 0.1-0.25% Sodium Deoxycholate vs 420mM NaCl in TrionX-100 lysis buffer. Which is Started by asaren , 18 May 2015 Co-IP, Cell Lysis	4 replies 1,767 views	asaren 19 May 2015
Protocols and Techniques Forums → Protein and Proteomics → Cell lysis method to get "intact" nuclear protein complex for CO-IP? Started by asaren , 13 May 2015 cell lysis, Co-IP and 1 more...	1 reply 2,260 views	bob1 14 May 2015
Protocols and Techniques Forums → Cell Biology → Does Ultrasonic probe sonication break either mitochondrial or nucler membrane? Started by asaren , 09 May 2015 cell lysis, cell fractation and 3 more...	3 replies 1,793 views	Curtis 09 May 2015
Protocols and Techniques Forums → Molecular Biology → Co-immunoprecipitation non-specific binding Started by tofuj , 14 Jan 2015 co-ip, co-immunoprecipitation	2 replies 1,395 views	tofuj 14 Jan 2015