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background staining in IHC ofartificial oralmucosa


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#1 kulasekara

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Posted 30 July 2004 - 04:09 AM

Hello,
I was trying to detect laminin 5 antigen in praffin embedded sections of artificially grown oral mucosa tissue(keratinocytes grown on fibroblasts enriched collagen 1 biomatrix) using DAKO envision kit.
i tried dilutions of polyclonal antilaminin 5 from 1:100 to 1:1000 still there is background staining.
can you suggest me how to overcome this?
keerthi

#2 ihcworld

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Posted 06 August 2004 - 09:44 AM

Check this link:

IHC Troubleshooting

#3 kulasekara

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Posted 27 August 2004 - 01:33 AM

hi,
Thanks. I got good results afterwards.
keerthi




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