Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
- - - - -


Pool down assay

  • Please log in to reply
1 reply to this topic

#1 Vijaytailor



  • Members
  • Pip
  • 2 posts

Posted 24 March 2015 - 09:05 PM

Hi there,

I am performing a pool down assay using ProteinS sepharose,  in that i am getting my protein of intereast, and Anti body also in the elute.

My problem is that i want to elute my protein but not the Antibody in it. Can any one suggest any protocol for this?

#2 mdfenko


    an elder emeritus

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 3,361 posts

Posted 25 March 2015 - 05:27 AM

you can crosslink the antibody to protein a sepharose (i've used protein a, you can also use protein g, never heard of protein s sepharose) prior to binding your protein of interest then elute with either low pH or high pH (whichever works better for you). the antibody will remain attached to the immobilized protein.

Edited by mdfenko, 25 March 2015 - 05:27 AM.

talent does what it can
genius does what it must
i used to do what i got paid to do

Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.