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Tab-seq or oxBS-seq for locus specific hmeC analysis

oxBS tab-seq hmeC

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#1 Galleg

Galleg

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Posted 23 March 2015 - 03:22 AM

Hi everybody,

Iperformed MeDip/hmedip with my samples and was able to identify some intersting regions which i would like to validate now in a single bp resultion. For methylated cytosine the approach is clear however for hmeC two methods are avaible and iam not really sure which on is better. I working with in vivo FAC-sorted cells so the amount of mateiral is a big issue therefor i would highly prefer a method were i loss less material. I can image the major drawback of oxBS is the cutting of the DNA into small pices due the two chemical conversions. However with TAB-seq iam not sure about the efficincy ?

 

Would be great if i would get some opinions from experts :)

 

Cheers,

Flo  






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