I have a problem about mutagenesis. I want to mutate 2 base pairs in plasmid,and use stratagenne mutagenesis protocol.Design 2 oligos as
primers.After I run PCR, I only see one band less than 200bp.It seems these two complementary oligos I designed connected together.But there is no mutant plasmid.and after I transform,there is no clony,
since these 2 oligos(primers) are complementary,how can I decrease
these 2 connect together and increase plasmid strand and primer connection?
thanks a lot:)
Edited by kroger, 29 July 2004 - 05:52 PM.