Google Sign in options
Remember me
This is not recommended for shared computers

Sign in anonymously
Don't add me to the active users list

Privacy Policy
Sign In
Create Account

Javascript Disabled Detected

You currently have javascript disabled. Several functions may not work. Please re-enable javascript to access full functionality.

Submit your paper to J Biol Methods today!

Fixation of Surface Antigen

Started by H. Aly, Mar 20 2015 03:31 AM

fixation extracellualr staining

Please log in to reply

No replies to this topic

#1 H. Aly

member

Active Members
15 posts

0
Neutral

Posted 20 March 2015 - 03:31 AM

I need your help. I want to stain a surface antigen, but I won´t be able to use the flow cytometer on the same day, I read that you can fix the cells with either ethanol, or formaldhyde ..etc . Which is better ? Will it affect the results ? I am using PE-conjugated antibody.

Back to Flow Cytometry

Also tagged with one or more of these keywords: fixation, extracellualr, staining

Protocols and Techniques Forums → Histology and Pathology → Antigen Retrieval Started by SAT142, 02 Jan 2020 histology, antigen retrieval and 2 more...	0 replies 1,553 views	SAT142 02 Jan 2020
Protocols and Techniques Forums → Flow Cytometry → Fixation for surface epitope detection Started by Thomson, 05 Nov 2018 flow cytometery, surface marker and 1 more...	1 reply 879 views	mdfenko 07 Nov 2018
Protocols and Techniques Forums → Protein and Proteomics → Membrane and whole cell staining Started by Mad Researcher, 07 Feb 2018 staining, immunohistochemistry and 2 more...	0 replies 1,653 views	Mad Researcher 07 Feb 2018
Protocols and Techniques Forums → Histology and Pathology → Intestine tissue falling of slides during H and E staining Started by bryant.keirns, 02 Feb 2018 histology, staining, tissue loss and 1 more...	1 reply 1,603 views	Rnotk 15 Feb 2018
Protocols and Techniques Forums → Tissue and Cell Culture → Neuron dead after immuno Started by Mad Researcher, 22 Jan 2018 immunocytochemistry and 3 more...	8 replies 1,426 views	Mad Researcher 24 Jan 2018