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Fixation of Surface Antigen

Started by H. Aly, Mar 20 2015 03:31 AM

fixation extracellualr staining

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#1 H. Aly

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Posted 20 March 2015 - 03:31 AM

I need your help. I want to stain a surface antigen, but I won´t be able to use the flow cytometer on the same day, I read that you can fix the cells with either ethanol, or formaldhyde ..etc . Which is better ? Will it affect the results ? I am using PE-conjugated antibody.

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