Posted 29 July 2004 - 08:59 AM
I need to do some electrophoresis of proteins. I have experience of nucleic acid electrophoresis, but have no experience about proteins. In some handbook I found that there are at least two types of SDS-PAGE of proteins, Tris-tricine buffer system (discontinuous) and continuous system. The latter seems to be simple, but I encountered several problems. The 4* phosphate/SDS buffer (0.4 M sodium phosphate/0.4% SDS, pH 7.2) is said to be able to be stored at 4C degree for up to 3 months, but in my hand the buffer crystallized after being put into the refrigerator (4C degree). Even under room temperature there are some crystals. And same thing happens with the 2*phosphate/SDS sample buffer (20 mM sodium phosphate, 2% SDS, 0.001% bromophenol blue, 0.2 M DTT, and 2% glycerol). I don't know whether I need to store these buffers under room temperature. Any suggestions would be really appreciated. Thanks a lot.