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Can I store media containing TPA (PMA) at 4 degrees?


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#1 SimoneM

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Posted 18 March 2015 - 05:39 PM

We use TPA (PMA) in our lab to differentiate neuroblastoma cell lines. We have found that 50ng/mL PMA works best.
 
We stock the chemical as 1mg/mL in I believe DMSO.
 
To get 50ng/mL I need to add 1uL of 1mg/mL TPA to each 20mL of media.
 
This just seems like a waste of media as I don't need so much right now. Also, I fear that the repeated Freeze/thaw cycles of the TPA might affect its stability.
 
I read that TPA is sensitive to light, so Im keeping its time out of the freezer limited, and I'll wrap the media in foil if I am able to store it at 4 degrees.
 
But can I store TPA containing media at 4 degrees Celsius, and for how long?

Edited by SimoneM, 18 March 2015 - 05:40 PM.


#2 KirstenOrion

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Posted 07 April 2015 - 09:50 AM

Contact the authors of this article. They could probably help.

 

Later, visit  the leader in 3D cell culture. Also cancer and neuroblastoma models: 






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