Edited by SimoneM, 18 March 2015 - 05:40 PM.
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Can I store media containing TPA (PMA) at 4 degrees?
1 reply to this topic
Posted 18 March 2015 - 05:39 PM
We use TPA (PMA) in our lab to differentiate neuroblastoma cell lines. We have found that 50ng/mL PMA works best.
We stock the chemical as 1mg/mL in I believe DMSO.
To get 50ng/mL I need to add 1uL of 1mg/mL TPA to each 20mL of media.
This just seems like a waste of media as I don't need so much right now. Also, I fear that the repeated Freeze/thaw cycles of the TPA might affect its stability.
I read that TPA is sensitive to light, so Im keeping its time out of the freezer limited, and I'll wrap the media in foil if I am able to store it at 4 degrees.
But can I store TPA containing media at 4 degrees Celsius, and for how long?