Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Where to obtain strain E. coli K12 SB536 ?

expression bacteria perisplasm

  • Please log in to reply
3 replies to this topic

#1 macrox

macrox

    member

  • Active Members
  • Pip
  • 9 posts
1
Neutral

Posted 18 March 2015 - 12:34 AM

Dear all,

 

does anyone know where to get the E. coli K-12 SB536 strain (good for expression of proteins in periplasm)? I searched the Google all over, however I did not get any useful clues.

 

Thanks!


Edited by macrox, 18 March 2015 - 03:25 AM.


#2 bob1

bob1

    Thelymitra pulchella

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 6,407 posts
517
Excellent

Posted 18 March 2015 - 01:23 AM

try looking in this article: http://www.ncbi.nlm....cles/PMC317789/



#3 macrox

macrox

    member

  • Active Members
  • Pip
  • 9 posts
1
Neutral

Posted 18 March 2015 - 03:24 AM

Thank you, but there is no mention of this strain. Actually, the primary citation is http://www.ncbi.nlm..../pubmed/8576052, however this (slightly old) paper is from people at Genentech.

It seems that this strain is not commercially available. Perhaps it can be obtained from some group?


Edited by macrox, 18 March 2015 - 03:25 AM.


#4 phage434

phage434

    Veteran

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 2,747 posts
304
Excellent

Posted 18 March 2015 - 04:51 AM

I would try the Yale E. coli Genetic Stock Center.

http://cgsc.biology.yale.edu







Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.