I have purified the histag protein from E.Coil. The purity is perfect, more than 98%,checked by Mass. But now I found a very strange things that the CD spectrum was different with other peoples data. And I measured the CD spectrum just before I did lyophilization. It is different with after lyophiliation but similar with others.
What happened during the lyophilization? Tris-tricin shows no any change after lyophilization!
Thanks in advance!
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conformation changed after lyophilizatin
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