I work in a very small and new lab focused on Trypanosoma cruzi (needless to say, with a very tight budget, which seems to be an epidemic lately).
We need to get the molecular part of the research center going (it has been focused on epidemiology research and chemistry). The molecular lab is a recent addition and in order to make it grow we need to have an ongoing project with the few things we have.
I basically have PCR and electrophoresis capabilities, a mass spec, sonicator, incubators and centrifuges. There is a rather "simple" project that focuses on new PCR methods to KO certain proteins to determine if they are essential or not (for future use as drug targets).
Problem is, we don't have an electroporator and I don't think we are getting one any time soon. I am trained in bacterial genetics and in some immunology, but I have no previous experience with protozoans.
So the question: is it possible to transfect trypanosomes chemically (i.e. calcium phosphate)? Or any other way that doesn't require equipment? I am willing to try chemical transfection, but I don't want to waste money we barely have in something that has been proven as ineffective.
Any suggestion (transfection methods or research with the few things I have) will be highly appreciated!