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contamination in HepG2 cell culture

Hepg2

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#1 attached to cells!

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Posted 09 March 2015 - 03:39 PM

Hi All,

 

I have been maintaining HepG2 cell culture. I split the cells on 1:4 ratio on Friday. I was out on the weekend and I came back on Monday to change the media. I noticed granulated extracellular particles. I initially thought it was cell debris but because of the sheer number I am concerned its bacterial contamination. Could you please give me your insights on it. I will truly appreciate it. Attached is the image.

 

Thanks!

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  • hepg2_03062015_passage number2_AS (4).jpg


#2 bob1

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Posted 10 March 2015 - 12:39 AM

Based on the size and regularity I would say yeast contamination.







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