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Amplification problem in DNA isolated after ChIP experiment,

ChIP RTPCR Chromatin

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#1 Rajan

Rajan

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Posted 08 March 2015 - 07:02 AM

I am trying to work out a ChIP experiment. After my ChIP experiment, the DNA I am getting in INPUT is showing problem with amplification. In an RT PCR experiment when I am taking 1ul of this INPUT DNA, It shows a CT value of 36. While on increasing the volume of INPUT dna to 4ul in RT PCR,  it does not even get amplified in 40 cycles. 

 

I am using diagenode Ipure kit for DNA isolation (it is working fine in my other experiments). Problem persisted even when I have purified this  INPUT DNA with agencourt amp pure beads. I have repeated the experiment 2 -3 times with same results. 

 

Any suggestions  !!

 

 

 

  







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