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I stored my PVDF membrane at 4C ?


Best Answer GNANA, 06 March 2015 - 10:45 AM

It shouldn't be a problem. you can continue from there.

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#1 msk1107

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Posted 06 March 2015 - 10:23 AM

Can anyone help me...?

I performed SDS and then did western blotting but  I didnt had time to finish my western blotting so I left it in blocking buffer containing 5% non fat dried milk in TBST. My question is that will my membrane be alright to blot on monday morning (i left it on friday) as I have seen in a few places that it wont be an issue but I just want to make sure as I dont want to blot and end up with nothing and then repeat the whole process again :/....?

 

 

Can a get a fast reply pleeeeeeasee!...

Thank youu



#2 GNANA

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Posted 06 March 2015 - 10:45 AM   Best Answer

It shouldn't be a problem. you can continue from there.


I would prefer being perfectionist rather than a passionist in Research.

I always had an alternate hypothesis....

#3 asaren

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Posted 09 May 2015 - 05:45 AM

You can left the membrane in blocking buffer at -20 degree C for months.






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