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Fixing cells in 70% Ethanol-ChIPSeq

ChIPSeq FACs Fixative

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#1 Teri

Teri

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Posted 04 March 2015 - 06:46 PM

Hello:

 

Im wondering does anybody have experience with this.

Im currently optimising for Mitotic ChIPSeq. My plan was to formaldehyde crosslink my samples, quench with glycine and store in 70% EtOH until I had enough cells to proceed with the FACs sorting and ChIPSeq.

Can anybody tell me if the EtOH will interfere with my ChIP???

 

Thanks!







Also tagged with one or more of these keywords: ChIPSeq, FACs, Fixative

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