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Fixing cells in 70% Ethanol-ChIPSeq

ChIPSeq FACs Fixative

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#1 Teri

Teri

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Posted 04 March 2015 - 06:46 PM

Hello:

 

Im wondering does anybody have experience with this.

Im currently optimising for Mitotic ChIPSeq. My plan was to formaldehyde crosslink my samples, quench with glycine and store in 70% EtOH until I had enough cells to proceed with the FACs sorting and ChIPSeq.

Can anybody tell me if the EtOH will interfere with my ChIP???

 

Thanks!







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